reference american type culture collection 19977  (ATCC)

Journal: The Journal of Infectious Diseases

Article Title: Phosphatidylinositol 5-Phosphate-Loaded Apoptotic Body-Like Liposomes for Mycobacterium abscessus Infection Management in Patients With Cystic Fibrosis

doi: 10.1093/infdis/jiaf124

Figure Lengend Snippet: ABL/PI5P reduces Mycobacterium abscessus intracellular viability in macrophages from PWCF irrespective of ETI therapeutic regimen eligibility. Monocyte-derived macrophages from PWCF under an ETI regimen (n = 15) ( A ) or from PWCF without a modulator regimen (n = 15) ( B ) were cultured at a concentration of 1 × 10 6 cells/mL in 96-well plates. Cells were then infected with M. abscessus at multiplicity of infection 10 for 3 hours and finally stimulated with ABL/PI5P and/or ETI for 18 hours. Replication index was calculated as the ratios between the colony-forming units obtained after 18 hours from infection, in the presence or absence of stimuli, and those obtained immediately after infection. For a deeper analysis of the results, patients with CF represented in ( B ) were divided into 2 groups: ( C ) those eligible (n = 8) and ( D ) not eligible (n = 7) for the ETI therapeutic regimen. The results are shown as mean ± standard deviation of the values obtained. Statistical analysis was performed by 2-sided Wilcoxon matched-pairs signed rank test. * P < .05, ** P < .01 *** P < .001, **** P < .0001. If not indicated by the line, the comparisons were performed versus control. Abbreviations: ABL/PI5P, apoptotic body-like liposome/phosphatidylinositol 5-phosphate; PWCF, people with cystic fibrosis; ETI, elexacaftor, tezacaftor, ivacaftor; ns, not significant.

Article Snippet: M. abscessus reference strain American Type Culture Collection (ATCC) 19977 and the M. abscessus subsp abscessus clinical strain Mab285 (previously referred to as CP07), a representative of the dominant clone 1 of M. abscessus subsp abscessus , were used [ ].

Techniques: Derivative Assay, Cell Culture, Concentration Assay, Infection, Standard Deviation, Control

Journal: The Journal of Infectious Diseases

Article Title: Phosphatidylinositol 5-Phosphate-Loaded Apoptotic Body-Like Liposomes for Mycobacterium abscessus Infection Management in Patients With Cystic Fibrosis

doi: 10.1093/infdis/jiaf124

Figure Lengend Snippet: ABL/PI5P-amikacin combined treatment reduces Mab285 intracellular viability in macrophages of PWCF who do not receive ETI therapeutic regimen. MDM from PWCF without a modulator regimen (n = 6) were cultured at a concentration of 1 × 10 6 cells/mL in 96-well plates. Cells were then infected with Mycobacterium abscessus at multiplicity of infection 10 for 3 hours and stimulated with ABL/PI5P and/or 4 µg/mL amikacin for 18 hours. Finally, supernatant was collected, and MDM were lysed to enumerate extracellular ( A ) and intracellular ( B ) bacteria. Replication index was calculated as the ratio between the colony-forming units obtained 18 hours after infection in the presence or absence of ABL/PI5P and/or amikacin, and those obtained immediately after infection and before the stimuli. The results are shown as mean ± standard deviation of the values obtained. Statistical analysis was performed by 2-sided Wilcoxon matched-pairs signed rank test. * P < .05. If not indicated by the line, the comparisons were performed versus control. Abbreviations: ABL/PI5P, apoptotic body-like liposome/phosphatidylinositol 5-phosphate; PWCF, people with cystic fibrosis; ETI, elexacaftor, tezacaftor, ivacaftor; MDM, monocyte-derived macrophages; ns, not significant.

Article Snippet: M. abscessus reference strain American Type Culture Collection (ATCC) 19977 and the M. abscessus subsp abscessus clinical strain Mab285 (previously referred to as CP07), a representative of the dominant clone 1 of M. abscessus subsp abscessus , were used [ ].

Techniques: Cell Culture, Concentration Assay, Infection, Bacteria, Standard Deviation, Control, Derivative Assay